| Peer-Reviewed

Poly (2-Hydroxyethyl Methacrylate) Macroporous Cryogel for Extracorporeal Medical Devices

Received: 5 August 2015     Accepted: 11 August 2015     Published: 19 August 2015
Views:       Downloads:
Abstract

Poly (2-hydroxyethyl methacrylate) PHEMA monolithic cryogels were synthesized by free radical polymerization at -12°C for 18 hours and produced spongy, elastic and macroporous gel matrix. Scanning Electron Microscopy (SEM) measured structural properties of PHEMA monolithic cryogel matrix to visualize pore morphology. Mechanical properties of PHEMA monolithic cryogel such as storage modulus, compressive modulus, and creep test were measured with Dynamic mechanical analyzer (DMA). The PHEMA monolithic cryogel matrix shows ~ 97% recovery after 70% compression of cryogel and has a compressive modulus of 1.8kPa to 8.5kPa.

Published in International Journal of Biomedical Materials Research (Volume 3, Issue 4)
DOI 10.11648/j.ijbmr.20150304.12
Page(s) 46-55
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Macroporous Cryogel, Poly (2-Hydroxyethyl Methacrylate), Mechanical Properties, Creep Test, Compressive Modulus

References
[1] Arvidsson, P., et al., Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns. Journal of Chromatography A, 2002. 977(1): p. 27-38.
[2] Plieva, F. M., et al., Pore structure in supermacroporous polyacrylamide based cryogels. Soft Matter, 2005. 1(4): p. 303-309.
[3] Dainiak, M. B., et al., Integrated isolation of antibody fragments from microbial cell culture fluids using supermacroporous cryogels. Journal of Chromatography A, 2004. 1045(1-2): p. 93-98.
[4] Arvidsson, P., et al., Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent. Journal of Chromatography A, 2003. 986(2): p. 275-90.
[5] Plieva, F. M., H. Kirsebom, and B. Mattiasson, Preparation of macroporous cryostructurated gel monoliths, their characterization and main applications. Journal of Separation Science, 2011. 34(16-17): p. 2164-2172.
[6] Bowers, R. W. J. and B. J. Tighe, Studies of the ocular compatibility of hydrogels. A review of the clinical manifestations of spoliation. Biomaterials, 1987. 8(2): p. 83-IN1.
[7] Rosiak, J., J. Olejniczak, and W. Pakala, Fast reaction of irradiated polymers. Crosslinking and degradation of polyvinylpyrrolidone. International Journal of Radiation Applications and Instrumentation. Part C. Radiation Physics and Chemistry, 1990. 36(6): p. 747-755.
[8] Dziubla, T. D., et al., Evaluation of porous networks of poly(2-hydroxyethyl methacrylate) as interfacial drug delivery devices. Biomaterials, 2001. 22(21): p. 2893-2899.
[9] Bajpai, A. K. and M. Shrivastava, Water sorption dynamics of a binary copolymeric hydrogel of 2-hydroxyethyl methacrylate (HEMA). Journal of Biomaterials Science, Polymer Edition, 2002. 13(3): p. 237-256.
[10] Lozinsky, V. I., et al., Polymeric cryogels as promising materials of biotechnological interest. Trends in Biotechnology, 2003. 21(10): p. 445-451.
[11] Savina, I. N., et al., Porous structure and water state in cross-linked polymer and protein cryo-hydrogels. Soft Matter, 2011. 7(9): p. 4276-4283.
[12] Ertürk, G. and B. Mattiasson, Cryogels-versatile tools in bioseparation. Journal of Chromatography A, 2014. 1357: p. 24-35.
[13] Gun'ko, V. M., I. N. Savina, and S. V. Mikhalovsky, Cryogels: morphological, structural and adsorption characterisation. Adv Colloid Interface Sci, 2013. 187-188: p. 1-46.
[14] Hanora, A., et al., Screening of peptide affinity tags using immobilised metal affinity chromatography in 96-well plate format. Journal of Chromatography A, 2005. 1087(1–2): p. 38-44.
[15] Le Noir, M., et al., Macroporous molecularly imprinted polymer/cryogel composite systems for the removal of endocrine disrupting trace contaminants. Journal of Chromatography A, 2007. 1154(1–2): p. 158-164.
[16] Ingavle, G. C., et al., Affinity binding of antibodies to supermacroporous cryogel adsorbents with immobilized protein A for removal of anthrax toxin protective antigen. Biomaterials, 2015. 50: p. 140-153.
[17] Hajizadeh, S. and B. Mattiasson, Cryogels with Affinity Ligands as Tools in Protein Purification, in Affinity Chromatography, S. Reichelt, Editor. 2015, Springer New York. p. 183-200.
[18] Plieva , F., et al., Macroporous elastic polyacrylamide gels prepared at subzero temperatures: control of porous structure. Journal of Materials Chemistry, 2006. 16(41): p. 4065-4073.
[19] Jones, D. S., et al., Pharmaceutical applications of dynamic mechanical thermal analysis. Advanced Drug Delivery Reviews, 2012. 64(5): p. 440-448.
[20] Jones, D. S., A. D. Woolfson, and A. F. Brown, Textural, viscoelastic and mucoadhesive properties of pharmaceutical gels composed of cellulose polymers. International Journal of Pharmaceutics, 1997. 151(2): p. 223-233.
[21] Ferry, J. D., Viscoelastic Properties of Polymers. 1980: John Wiley and Sons.
[22] Ward , I. M. and D. W. Hardley An introduction to the mechanical properties of solid polymers. 1993: John Wiley and sons.
[23] Jones, D. S., Dynamic mechanical analysis of polymeric systems of pharmaceutical and biomedical significance. International Journal of Pharmaceutics, 1999. 179(2): p. 167-178.
[24] Barnes, H. A., J. F. Hutton, and K. Walters, An introduction to rheology. 1996: Elsevier.
[25] Craig, D. Q. M. and F. A. Johnson, Pharmaceutical applications of dynamic mechanical thermal analysis. Thermochimica Acta, 1995. 248: p. 97-115.
[26] Patfoort, G., An introduction to physical , mechanical and rheological behaviour. 1974: Story -Scientia , Gent.
[27] Denizli, A., R. Say, and E. Piskin, Removal of aluminium by Alizarin Yellow-attached magnetic poly(2-hydroxyethyl methacrylate) beads. Reactive and Functional Polymers, 2003. 55(1): p. 99-107.
[28] Hollister, S. J., Porous scaffold design for tissue engineering. Nature Materials, 2005. 4(7): p. 518-524.
[29] Dainiak, M. B., et al., Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel. Proceedings of the National Academy of Sciences, 2006. 103(4): p. 849-854.
[30] Casahoursat, L., G. Lemagnen, and D. Larrouture, The Use of Stress Relaxation Trials to Characterize Tablet Capping. Drug Development and Industrial Pharmacy, 1988. 14(15-17): p. 2179-2199.
Cite This Article
  • APA Style

    Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. (2015). Poly (2-Hydroxyethyl Methacrylate) Macroporous Cryogel for Extracorporeal Medical Devices. International Journal of Biomedical Materials Research, 3(4), 46-55. https://doi.org/10.11648/j.ijbmr.20150304.12

    Copy | Download

    ACS Style

    Wuraola Akande; Lyuba Mikhalovska; Stuart James; Sergey Mikhalovsky. Poly (2-Hydroxyethyl Methacrylate) Macroporous Cryogel for Extracorporeal Medical Devices. Int. J. Biomed. Mater. Res. 2015, 3(4), 46-55. doi: 10.11648/j.ijbmr.20150304.12

    Copy | Download

    AMA Style

    Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. Poly (2-Hydroxyethyl Methacrylate) Macroporous Cryogel for Extracorporeal Medical Devices. Int J Biomed Mater Res. 2015;3(4):46-55. doi: 10.11648/j.ijbmr.20150304.12

    Copy | Download

  • @article{10.11648/j.ijbmr.20150304.12,
      author = {Wuraola Akande and Lyuba Mikhalovska and Stuart James and Sergey Mikhalovsky},
      title = {Poly (2-Hydroxyethyl Methacrylate) Macroporous Cryogel for Extracorporeal Medical Devices},
      journal = {International Journal of Biomedical Materials Research},
      volume = {3},
      number = {4},
      pages = {46-55},
      doi = {10.11648/j.ijbmr.20150304.12},
      url = {https://doi.org/10.11648/j.ijbmr.20150304.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20150304.12},
      abstract = {Poly (2-hydroxyethyl methacrylate) PHEMA monolithic cryogels were synthesized by free radical polymerization at -12°C for 18 hours and produced spongy, elastic and macroporous gel matrix. Scanning Electron Microscopy (SEM) measured structural properties of PHEMA monolithic cryogel matrix to visualize pore morphology. Mechanical properties of PHEMA monolithic cryogel such as storage modulus, compressive modulus, and creep test were measured with Dynamic mechanical analyzer (DMA). The PHEMA monolithic cryogel matrix shows ~ 97% recovery after 70% compression of cryogel and has a compressive modulus of 1.8kPa to 8.5kPa.},
     year = {2015}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Poly (2-Hydroxyethyl Methacrylate) Macroporous Cryogel for Extracorporeal Medical Devices
    AU  - Wuraola Akande
    AU  - Lyuba Mikhalovska
    AU  - Stuart James
    AU  - Sergey Mikhalovsky
    Y1  - 2015/08/19
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ijbmr.20150304.12
    DO  - 10.11648/j.ijbmr.20150304.12
    T2  - International Journal of Biomedical Materials Research
    JF  - International Journal of Biomedical Materials Research
    JO  - International Journal of Biomedical Materials Research
    SP  - 46
    EP  - 55
    PB  - Science Publishing Group
    SN  - 2330-7579
    UR  - https://doi.org/10.11648/j.ijbmr.20150304.12
    AB  - Poly (2-hydroxyethyl methacrylate) PHEMA monolithic cryogels were synthesized by free radical polymerization at -12°C for 18 hours and produced spongy, elastic and macroporous gel matrix. Scanning Electron Microscopy (SEM) measured structural properties of PHEMA monolithic cryogel matrix to visualize pore morphology. Mechanical properties of PHEMA monolithic cryogel such as storage modulus, compressive modulus, and creep test were measured with Dynamic mechanical analyzer (DMA). The PHEMA monolithic cryogel matrix shows ~ 97% recovery after 70% compression of cryogel and has a compressive modulus of 1.8kPa to 8.5kPa.
    VL  - 3
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK

  • Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UKBiomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK

  • Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK

  • Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK

  • Sections