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Beta-Lactamase Production Among Uropathogens in Patients Attending Some Hospitals in Kano, Nigeria

Received: 19 August 2016     Accepted: 2 December 2016     Published: 4 January 2017
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Abstract

Ability of bacteria to withstand antibiotic therapy is often associated with resistance. Certain bacteria have the ability to produce some enzymes, like Beta–Lactamase, which confers resistance to some groups of antibiotics like penicillins and cephalosporins. The study focused on producers of these enzymes among uropathogens. A total of one hundred and forteen isolates (114) were screened, out of which seventy one 71 (62.3%) were found positive for the enzymes. Eschericia coli had the highest positivity rate 40 (56.3%). Followed by Klebsiella spp., Staphylococcus aureus, Staphylociccus saprophyticcus, Proteus mirabilis, and Pseudomonas aeruginosa with 15 (21.1%), 7 (10.0%), 5 (7.0%), 3 (4.2%), and 1 (1.4%) respectively. In the same vein, it was discovered that, isolates of females’ origin, produced more of the enzymes than those of their males counterpart. This was indicated by the production of the enzyme by at least one isolate in each of the species isolated from female samples while in males, some species did not even produce any of the enzymes. Age-wise distribution of the enzymes showed that, 21- 30, and 31-40 age groups had all the isolates producing the enzymes in varying frequencies. Only Pseudomonas aeruginosa that produced its enzyme outside these age groups. Isolates of females origin had more of the enzymes produced in all the species compared to those from males, with ratios of; 26:14, 11:4, 5:2, 3:2, 2:1, and 1:0 for, E coli, Klebsiella species, Staphylococcus aureus, Staphylococcus saprophyticus, Proteus mirabilis, and Pseudomonas aeruginosa respectively.

Published in International Journal of Biomedical Materials Research (Volume 4, Issue 3)
DOI 10.11648/j.ijbmr.20160403.18
Page(s) 58-62
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Urinary Tract Infections, Uropathogens, Beta-Lactamase, Kano

References
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[2] Alabi, O. S., Onyenwe, N. E. Satoye, K. A. and Adeleke, O. E. (2014). Prevalence of extended-spectrum β-lactamase producing isolates from asymptomatic bacteriuria among students in a tertiary institution in Ibadan, Nigeria. Nature and Science 12 (4): 113.
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[4] Ben–Ami, R., Rodriguez–Bafio, J., Calbo E. S. And Arslan, H. A. (2009). Multinational survey of risk factors for infection with extended spectrum beta lactamasesmproducing Enterobacteriaceae in non hospitalized patients. 5 (1): 49–51.
[5] Bush, K., Jacoby, G. A. and Medeiros, A. A. (1995). A Functional Classification Scheme for β- lactamases and its Correlation with Molecular Structure. Antimicrob. Agents Chemother. 39: 1211-33.
[6] Chaliha, C. Stanton, S. L., (2002). Urological problems in pregnancy. BJU Int., 89 (5): 469-76. To remove
[7] Chander and Shrestha, (2013). Prevalence of extended spectrum beta lactamase producing Escherichia coli and Klebsiella pneumoniae urinary isolates in a tertiary care hospital in Kathmandu, Nepal. BMC Research Notes 6: 487.
[8] Cheesebrough, M. (2000). Distric Laboratory Practice in Tropical Countries Low Price ed. Chambridge University Press. Pp. 434.
[9] Cox, C. E. (1988). Nosocomial urinary tract infections. Urol, 32 (3): 210–215.
[10] Datta, N. and Kontomichalou, P. (1965)."Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae." Nature 208: 239-41.
[11] Foxman, B., Barlow, R., D’Arcy, H., Gillespie, B., and Sobel, J. D. (2000). Urinary tract infection: Self-reported incidence and associated costs. Ann Epidemiol; 10 (8): 509-15.
[12] Gaurav, Dalela (2012). PREV. of Extended Spectrum Beta-Lactamase (ESBL) Producers among Gram Negative Bacilli from Various Clinical Isolates in a Tertiary Care Hospital at Jhalawar, Rajasthan, India. Journal of Clinical and Diagnostic Research. 6 (2): 182-187.
[13] Gonzalez, C. M., and Schaeffer, A. J. (1999). Treatment of urinary tract infection: what’s old, what’s new, and what works. World J Urol.; 17 (6): 372–382.
[14] Kapur, A., Ahmed, M. S., and John, S. (2015). Prevalence of Extended-Spectrum Beta-Lactamase-Producing Pathogens From Urinary Tract Infected Samples and Their Sensitivity Pattern Against Withania somnifera. Int J Infect. 2 (1): e22664.
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[17] Mansour, A. Manijeh, M. and Zohreh, P. (2009). Study of bacteria isolated from urinary tract infections and determination of their susceptibility to antibiotics. Jundishapur J Microbiol; 2 (3): 118–23.
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  • APA Style

    Hamza Sule, Abdulhadi Sale Kumurya. (2017). Beta-Lactamase Production Among Uropathogens in Patients Attending Some Hospitals in Kano, Nigeria. International Journal of Biomedical Materials Research, 4(3), 58-62. https://doi.org/10.11648/j.ijbmr.20160403.18

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    ACS Style

    Hamza Sule; Abdulhadi Sale Kumurya. Beta-Lactamase Production Among Uropathogens in Patients Attending Some Hospitals in Kano, Nigeria. Int. J. Biomed. Mater. Res. 2017, 4(3), 58-62. doi: 10.11648/j.ijbmr.20160403.18

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    AMA Style

    Hamza Sule, Abdulhadi Sale Kumurya. Beta-Lactamase Production Among Uropathogens in Patients Attending Some Hospitals in Kano, Nigeria. Int J Biomed Mater Res. 2017;4(3):58-62. doi: 10.11648/j.ijbmr.20160403.18

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  • @article{10.11648/j.ijbmr.20160403.18,
      author = {Hamza Sule and Abdulhadi Sale Kumurya},
      title = {Beta-Lactamase Production Among Uropathogens in Patients Attending Some Hospitals in Kano, Nigeria},
      journal = {International Journal of Biomedical Materials Research},
      volume = {4},
      number = {3},
      pages = {58-62},
      doi = {10.11648/j.ijbmr.20160403.18},
      url = {https://doi.org/10.11648/j.ijbmr.20160403.18},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20160403.18},
      abstract = {Ability of bacteria to withstand antibiotic therapy is often associated with resistance. Certain bacteria have the ability to produce some enzymes, like Beta–Lactamase, which confers resistance to some groups of antibiotics like penicillins and cephalosporins. The study focused on producers of these enzymes among uropathogens. A total of one hundred and forteen isolates (114) were screened, out of which seventy one 71 (62.3%) were found positive for the enzymes. Eschericia coli had the highest positivity rate 40 (56.3%). Followed by Klebsiella spp., Staphylococcus aureus, Staphylociccus saprophyticcus, Proteus mirabilis, and Pseudomonas aeruginosa with 15 (21.1%), 7 (10.0%), 5 (7.0%), 3 (4.2%), and 1 (1.4%) respectively. In the same vein, it was discovered that, isolates of females’ origin, produced more of the enzymes than those of their males counterpart. This was indicated by the production of the enzyme by at least one isolate in each of the species isolated from female samples while in males, some species did not even produce any of the enzymes. Age-wise distribution of the enzymes showed that, 21- 30, and 31-40 age groups had all the isolates producing the enzymes in varying frequencies. Only Pseudomonas aeruginosa that produced its enzyme outside these age groups. Isolates of females origin had more of the enzymes produced in all the species compared to those from males, with ratios of; 26:14, 11:4, 5:2, 3:2, 2:1, and 1:0 for, E coli, Klebsiella species, Staphylococcus aureus, Staphylococcus saprophyticus, Proteus mirabilis, and Pseudomonas aeruginosa respectively.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Beta-Lactamase Production Among Uropathogens in Patients Attending Some Hospitals in Kano, Nigeria
    AU  - Hamza Sule
    AU  - Abdulhadi Sale Kumurya
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    DO  - 10.11648/j.ijbmr.20160403.18
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    JF  - International Journal of Biomedical Materials Research
    JO  - International Journal of Biomedical Materials Research
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    AB  - Ability of bacteria to withstand antibiotic therapy is often associated with resistance. Certain bacteria have the ability to produce some enzymes, like Beta–Lactamase, which confers resistance to some groups of antibiotics like penicillins and cephalosporins. The study focused on producers of these enzymes among uropathogens. A total of one hundred and forteen isolates (114) were screened, out of which seventy one 71 (62.3%) were found positive for the enzymes. Eschericia coli had the highest positivity rate 40 (56.3%). Followed by Klebsiella spp., Staphylococcus aureus, Staphylociccus saprophyticcus, Proteus mirabilis, and Pseudomonas aeruginosa with 15 (21.1%), 7 (10.0%), 5 (7.0%), 3 (4.2%), and 1 (1.4%) respectively. In the same vein, it was discovered that, isolates of females’ origin, produced more of the enzymes than those of their males counterpart. This was indicated by the production of the enzyme by at least one isolate in each of the species isolated from female samples while in males, some species did not even produce any of the enzymes. Age-wise distribution of the enzymes showed that, 21- 30, and 31-40 age groups had all the isolates producing the enzymes in varying frequencies. Only Pseudomonas aeruginosa that produced its enzyme outside these age groups. Isolates of females origin had more of the enzymes produced in all the species compared to those from males, with ratios of; 26:14, 11:4, 5:2, 3:2, 2:1, and 1:0 for, E coli, Klebsiella species, Staphylococcus aureus, Staphylococcus saprophyticus, Proteus mirabilis, and Pseudomonas aeruginosa respectively.
    VL  - 4
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Author Information
  • Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria

  • Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria

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