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Evaluation of Antimicrobial Activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter Pylori Isolated from Ulcer Patients in Ondo State, Nigeria

Received: 13 February 2019     Accepted: 23 April 2019     Published: 23 May 2019
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Abstract

Antimicrobial activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter pylori isolated from ulcer patients in Ondo State, Nigeria were investigated. Hot water, cold water and two organic solvents (Pet ether and Ethanol) were used for the extraction with the highest yield being the ethanolic extracts of Carica papaya with 17.2%, while cold water extract of both Carica papaya and Azadirachta indica (Neem) extracts had 15.6% and 11.6% respectively. The result shows that the Carica papaya, Azadirachta indica and Moringa oleifera leaves extract had antimicrobial effect on the H. pylori isolated from ulcer patients in Ondo State, Nigeria. Moringa oleifera leaves showed some remarkable effect on H. pylori with the ethanolic extract of Carica papaya being the most potent. In comparison, the ethanolic extract had the highest level of antimicrobial activity than pet ether, cold and hot water extracts. The results of this study showed that leaf extracts of C. papaya, A. indica and M. oleifera had a very high microbial action on the isolates. The results of this study clearly suggest that leaf extracts of C. papaya, A. indica and M. oleifera leaves act as potent growth inhibitor of H. pylori. The emergence of resistance of microorganism to current first line therapy required vigorous research for substitute antimicrobial. The plants used in this study could serve as qualified plants in drug production. There is need for vigorous research to be done on the purification of these crude extracts for the development of new antibiotics to combat the infections caused by these resistant strains.

Published in International Journal of Biomedical Materials Research (Volume 7, Issue 1)
DOI 10.11648/j.ijbmr.20190701.18
Page(s) 61-66
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Antimicrobial, Carica Papaya, Azadirachta Indica, Moringa Oleifera, Helicobacter Pylori, Ulcer

References
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[4] Sabira, S., Muhammad, A., Hafiz, M. A. and Naveed, A. (2014). Complementary and alternative approaches to treat peptic ulcer. Int. Res. J. Pharm, 5 (5): 353-359
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[9] Debnath, S., Ray, K. and Guha, D. (2015). Effect of Moringa oleifera leaf extract on aspirin induced gastric mucosaldamage: Possible role of superoxide dismutase and catalase. Biomedine, 25 (2): 22-27.
[10] Siddhuraju, P. and Becker, K. J. (2013). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agro climatic origin of drumstick tree (Moringa oleifera Lam.) leaves. Agric Food Chem, 51 (8): 2144-2155.
[11] Tandon, R., Khanna, H. D., Dorababu, M. and Goel, R. K (2018). Oxidative stress and antioxidants ststus in peptic ulcer and gastric carcinoma. Indian J Physiology and Pharmacology, 48 (1): 115-118.
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[16] Ogundare, A. O. and Onifade, A. K. (2009). The antimicrobial activity of Moringa leaf extract on Escherichia coli. Journal of Medicinal Plant Research, 3 (4): 319 – 323.
[17] Cheesbrough, M. (2000). District Laboratory Practice in Tropical Countries, 2nd edition, Cambridge University press, Cambridge University Press, New York, Pp. 132-136.
[18] Smith, S. I., Emmanuel, A., Omonigbehin, H. A., Goodluck, F. B., Abdulkareem, C. A., Onyekwere, C. A., Dennis A. N., Muinah A. F., Jesse A. O., Monica C., Rainer H. and Gabriele, R. (2010). Diagnostic methods for the detection of Helicobacter pylori in Nigeria. T ropical Gastroenterology, 31 (2): 113 -115.
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[20] Kordali, S., Kotan, R., Mavi, A., Cakir, A., Ala, A. and Yildirim, A. (2018). Determination of chemical composition and antioxidant activity ofArtemisia dracunculus, and of the antifungal and antibacterial activities of Turkish Artemisisaabsinthium, Artemisiadrancunculus, Artemisia santonicum and Artemisia spicigera essential oils. Journal of Agricultural andfood Chemistry, 53: 9452-8.
[21] Srinivasan, D., Perumalsamy L., Nathan, S. and Sures, T. (2017). Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. Journal of Ethnopharmacology, 94: 12-14.
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    Morufu Adesina Bakare, Anthony Kayode Onifade. (2019). Evaluation of Antimicrobial Activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter Pylori Isolated from Ulcer Patients in Ondo State, Nigeria. International Journal of Biomedical Materials Research, 7(1), 61-66. https://doi.org/10.11648/j.ijbmr.20190701.18

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    ACS Style

    Morufu Adesina Bakare; Anthony Kayode Onifade. Evaluation of Antimicrobial Activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter Pylori Isolated from Ulcer Patients in Ondo State, Nigeria. Int. J. Biomed. Mater. Res. 2019, 7(1), 61-66. doi: 10.11648/j.ijbmr.20190701.18

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    AMA Style

    Morufu Adesina Bakare, Anthony Kayode Onifade. Evaluation of Antimicrobial Activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter Pylori Isolated from Ulcer Patients in Ondo State, Nigeria. Int J Biomed Mater Res. 2019;7(1):61-66. doi: 10.11648/j.ijbmr.20190701.18

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  • @article{10.11648/j.ijbmr.20190701.18,
      author = {Morufu Adesina Bakare and Anthony Kayode Onifade},
      title = {Evaluation of Antimicrobial Activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter Pylori Isolated from Ulcer Patients in Ondo State, Nigeria},
      journal = {International Journal of Biomedical Materials Research},
      volume = {7},
      number = {1},
      pages = {61-66},
      doi = {10.11648/j.ijbmr.20190701.18},
      url = {https://doi.org/10.11648/j.ijbmr.20190701.18},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20190701.18},
      abstract = {Antimicrobial activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter pylori isolated from ulcer patients in Ondo State, Nigeria were investigated. Hot water, cold water and two organic solvents (Pet ether and Ethanol) were used for the extraction with the highest yield being the ethanolic extracts of Carica papaya with 17.2%, while cold water extract of both Carica papaya and Azadirachta indica (Neem) extracts had 15.6% and 11.6% respectively. The result shows that the Carica papaya, Azadirachta indica and Moringa oleifera leaves extract had antimicrobial effect on the H. pylori isolated from ulcer patients in Ondo State, Nigeria. Moringa oleifera leaves showed some remarkable effect on H. pylori with the ethanolic extract of Carica papaya being the most potent. In comparison, the ethanolic extract had the highest level of antimicrobial activity than pet ether, cold and hot water extracts. The results of this study showed that leaf extracts of C. papaya, A. indica and M. oleifera had a very high microbial action on the isolates. The results of this study clearly suggest that leaf extracts of C. papaya, A. indica and M. oleifera leaves act as potent growth inhibitor of H. pylori. The emergence of resistance of microorganism to current first line therapy required vigorous research for substitute antimicrobial. The plants used in this study could serve as qualified plants in drug production. There is need for vigorous research to be done on the purification of these crude extracts for the development of new antibiotics to combat the infections caused by these resistant strains.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Evaluation of Antimicrobial Activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter Pylori Isolated from Ulcer Patients in Ondo State, Nigeria
    AU  - Morufu Adesina Bakare
    AU  - Anthony Kayode Onifade
    Y1  - 2019/05/23
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    N1  - https://doi.org/10.11648/j.ijbmr.20190701.18
    DO  - 10.11648/j.ijbmr.20190701.18
    T2  - International Journal of Biomedical Materials Research
    JF  - International Journal of Biomedical Materials Research
    JO  - International Journal of Biomedical Materials Research
    SP  - 61
    EP  - 66
    PB  - Science Publishing Group
    SN  - 2330-7579
    UR  - https://doi.org/10.11648/j.ijbmr.20190701.18
    AB  - Antimicrobial activities of Carica papaya, Azadirachta indica and Moringa oleifera on Helicobacter pylori isolated from ulcer patients in Ondo State, Nigeria were investigated. Hot water, cold water and two organic solvents (Pet ether and Ethanol) were used for the extraction with the highest yield being the ethanolic extracts of Carica papaya with 17.2%, while cold water extract of both Carica papaya and Azadirachta indica (Neem) extracts had 15.6% and 11.6% respectively. The result shows that the Carica papaya, Azadirachta indica and Moringa oleifera leaves extract had antimicrobial effect on the H. pylori isolated from ulcer patients in Ondo State, Nigeria. Moringa oleifera leaves showed some remarkable effect on H. pylori with the ethanolic extract of Carica papaya being the most potent. In comparison, the ethanolic extract had the highest level of antimicrobial activity than pet ether, cold and hot water extracts. The results of this study showed that leaf extracts of C. papaya, A. indica and M. oleifera had a very high microbial action on the isolates. The results of this study clearly suggest that leaf extracts of C. papaya, A. indica and M. oleifera leaves act as potent growth inhibitor of H. pylori. The emergence of resistance of microorganism to current first line therapy required vigorous research for substitute antimicrobial. The plants used in this study could serve as qualified plants in drug production. There is need for vigorous research to be done on the purification of these crude extracts for the development of new antibiotics to combat the infections caused by these resistant strains.
    VL  - 7
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Author Information
  • Department of Microbiology, The Federal University of Technology, Akure, Ondo State, Nigeria

  • Department of Microbiology, The Federal University of Technology, Akure, Ondo State, Nigeria

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